The method of degumming jute fibres with complex enzyme (2)

ABSTRACT

A method of degumming jute fibres with complex enzyme, wherein said complex enzyme comprises pectinase and laccase, comprises the steps of: a. Soaking the jute fibres in said complex enzyme water solution, wherein the weight proportion of said complex enzyme water solution and jute fibres ranges from 12:1 to 40:1; b. adjusting the PH value of said complex enzyme water solution to above 4.0, but below 5.0, and adjusting the temperature of said complex enzyme water solution to 35° C.-65° C., then keeping said complex enzyme water solution at such temperature for 20-120 minutes; c. adjusting the PH value of said complex enzyme water solution to 7.5-9.5, and adjusting the temperature of said complex enzyme water solution to 40° C.-70° C.; then, keeping said complex enzyme water solution with such temperature value for 20-120 minutes; d. conducting enzyme deactivation of the jute fibres processed with said complex enzyme.

TECHNICAL FIELD

The present invention relates to a method of degumming jute fibres, inparticular, relates to a method of degumming jute fibres with complexenzyme.

BACKGROUND

Bast fabrics have gained more and more popularity with people, due tobetter Moisture absorption & Breathing, Low electrostaticsusceptibility, and the antibacterial strength of bast fibres. Formaking the bast fabrics, the materials adopted can mainly be linenfibre, and ramie fibre, or the fibre combination of said fibres withother fibres, such as cotton fibres, wool fibres, chemical fibres, silkfibres after being blended spun. Linen or ramie is expensive, and thisis also the reason why the bast-fabric clothing has not been appliedwidely. However, Jute, which is cheaper than linen and ramie, has betterhygroscopicity and drapability than linen and ramie, and also has greatantibiotic ability. Therefore, jute has huge potentiality andapplication value in clothing making industry. As the content of ligninwithin jute is relatively high (reaching 10-13%), which is several timesas much as that within linen, it is not effective to degum jute fibresand remove the lignin from jute by using the existing degummingtechnology. And this greatly restrains the application of jute in makingclothing. <The Effect of Enzyme Treatment on Jute fibres> published in<<Jounal of Tianjin Industrial University>> volume 24 of August 2005introduces the effect of cellulose, hemicellulase, ligninase and pectindepolymerise used in processing the jute fibres, but this article onlyintroduces the method of processing jute fibres with single one of abovementioned enzymes. Although, there are some paragraphs in which themethods of complex enzyme treatment are mentioned, it only refers to thecomplex enzyme obtained via mixing laccase and cellulose enzyme ormixing hemicellulase enzyme and cellulose enzyme. However, it istestified in practice that it is not effective to remove lignin fromjute fibres using the degumming method published in this article.Chinese Patent publication No CN 1232691C introduces a method ofdegumming jute fibres with complex enzyme. In the method, pectinase andlaccase are used to produce a complex enzyme for degumming jute fibres,and the degummed jute fibres, after blended spun or interlaced withother fibres such as cotton fibres and chemical fibres, can generallymeet the requirements for clothing materials. However, the effect ofremoving lignin from jute fibres in the method, is still not goodenough, as the removal rate is only about 76%. The content of ligninremaining in the jute fibres is still very high, there is a need ofblended spinning or interlacing jute fibres with other fibres such ascotton fibres, and chemical fibres, when making the clothing materials.However, the quality of clothing materials made through blended spinningor interlacing jute fibres with other fibres such as cotton fibres, andchemical fibres still needs to be improved.

BRIEF DESCRIPTION OF INVENTION

The present invention relates to an effective method of degumming jutefibres with complex enzyme to remove pectin and lignin from said jutefibres.

In the present invention, a method of degumming jute fibres with complexenzyme, wherein said complex enzyme comprises pectinase and laccase,comprises the steps of:

a. soaking the jute fibres in the water solution of said complex enzymemade from pectinase and laccase, wherein the weight proportion of saidcomplex enzyme water solution and jute fibres ranges from 12:1 to 40:1;

b. adjusting the PH value of said complex enzyme water solution to above4.0, but below 5.0, and adjusting the temperature of said complex enzymewater solution to 35° C.-65° C., then keeping said complex enzyme watersolution at such temperature for 20-120 minutes;

c. adjusting the PH value of said complex enzyme water solution to7.5-9.5, and adjusting the temperature of said complex enzyme watersolution to 40° C.-70° C.; then, keeping said complex enzyme watersolution with such temperature value for 20-120 minutes;

d. conducting enzyme deactivation of the jute fibres processed with saidcomplex enzyme.

The method, wherein said jute fibres are accumulation stored before thestep d.

The method, wherein the duration for accumulation storing said jutefibres ranges from 6 to 24 hours.

The method, wherein the enzyme deactivation of jute fibres in the step dis through washing with hot water or adjusting the PH value of jutefibres, or through the combination of the two means.

The method, wherein the weight percentage of pectinase in said complexenzyme ranges from 30% to 90%.

The method, wherein the weight proportion of said complex enzyme andjute fibres ranges from 0.5:100 to 5:100.

The method, wherein the temperature of said hot water is above 75° C.;the PH value of jute fibres is adjusted to above 10.0 or below 4.0.

The method, wherein said jute fibres is pre-processed before the step a.The method, wherein the pre-processing of said jute fibres is eitherthrough one of the means of water bath, acid bath, and soaking withhydrogen Peroxide, or through the combination of at least two of thethree means.

The method, wherein that the temperature of water bath ranges from 30°C. to 100° C.; Said acid is sulphuric acid or acetic acid.

This invention also provides another method of degumming jute fibreswith complex enzyme, wherein said complex enzyme comprises pectinase andlaccase, said method comprises the steps of:

a. soaking the jute fibres in said complex enzyme water solution,wherein the weight proportion of said complex enzyme water solution andjute fibres ranges from 12:1 to 40:1;

b. adjusting the PH value of said complex enzyme water solution to5.0-5.5, and adjusting the temperature of said complex enzyme watersolution to 55° C.-60° C., then keeping said complex enzyme watersolution at such temperature for 51-120 minutes;

c. adjusting the PH value of said complex enzyme water solution to7.5-8.0, and adjusting the temperature of said complex enzyme watersolution to 60° C.-70° C.; then, keeping said complex enzyme watersolution with such temperature value for 20-120 minutes;

d. conducting enzyme deactivation of the jute fibres processed with saidcomplex enzyme.

The method, wherein said jute fibres are accumulation stored before thestep d.

The method, wherein the duration for accumulation storing said jutefibres ranges from 6 to 24 hours.

The method, wherein the enzyme deactivation of jute fibres in the step dis through washing with hot water or adjusting the PH value of jutefibres, or through the both of the two means.

The method, wherein the weight percentage of pectinase in said complexenzyme ranges from 30% to 90%.

The method, wherein the weight proportion of said complex enzyme andjute fibres ranges from 0.5:100 to 5:100.

The method, wherein the temperature of said hot water is above 75° C.;the PH value of jute fibres is adjusted to above 10.0 or below 4.0.

The method, wherein said jute fibres is pre-processed before the step a.

The method, wherein pre-processing said jute fibres is either throughone of the means of water bath, acid bath, and soaking with HydrogenPeroxide, or through at least two of the three means.

The method, wherein the temperature of water bath ranges from 30° C. to100° C.; said acid is sulphuric acid or acetic acid.

In comparison with the prior art, the present invention has severaladvantages as follows:

-   -   (1) In the present invention, the process parameters that match        with each other are used in treatment of degumming jute fibres        using complex enzyme. For example, Laccase is in the highest        activity when its PH value reaches about 4.5; adjusting the PH        value of enzyme water solution to below 5.0 or keeping the        enzyme water solution with a PH value that makes the enzyme        water solution in a relatively high activity, prolonging the        holding time of the enzyme water solution up to 50 minutes or        more, and accordingly adjusting other process parameters, in        order to gain the best effect of removing lignin. In addition,        it is effective to remove pectin and lignin from jute fibres        through accumulation storing the jute fibres before conducting        enzyme deactivation of the jute fibres treated with complex        enzyme via washing such jute fibres with hot water, or adjusting        the PH value of such jute fibres, The removal rate of pectin can        general reach about 90%, even up to 96% as the highest value,        while the removal rate of lignin can generally reach about 78%,        even up to 86% as the highest value. The jute fibres treated        through above mentioned method have relatively high spinability.    -   (2) In addition, pre-processing jute fibres before being        degummed can swell the jute fibres, so as to better reduce the        interacting force among the single fibres, facilitate the        contact between enzyme water solution and jute fibres, and        remove the pectin and lignin from the jute fibres.

DETAILED DESCRIPTION OF INVENTION Example 1

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through water bath at the temperature of 65, and the holdingtime is 2 hours; then, mixing the pectinase and laccase into complexenzyme, wherein the weight proportion of pectinase and laccase is 3:7,and the weight proportion of such complex enzyme and the jute fibres is0.5:100; next, diluting the complex enzyme with water, in order toproduce complex enzyme water solution which is 12 times in weight asmuch as jute fibres; after that, soaking the jute fibres in the dilutedcomplex enzyme water solution; then, adjusting the PH value of thediluted complex enzyme water solution to 4.4 with acetic acid and sodiumbicarbonate; next, heating the complex enzyme water solution to 35° C.and keeping the solution at such temperature for 20 minutes; after that,adjusting the PH value of the heated solution to 7.5 with sodiumbicarbonate, heating the solution to 70° C., and keeping the solution atsuch temperature for 20 minutes; then, taking the jute fibres out of thesolution, and accumulation storing the jute fibres for 24 hours; next,conducting enzyme deactivation of the accumulation stored jute fibres bywashing the jute fibres with hot water at 80° C.; finally, the degummedjute fibres are obtained. The removal rate of pectin and lignin fromjute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 2

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through both acid bath and water bath, wherein the acid usedfor acid bath is concentrated sulphuric acid with the concentration ofabove 90%. The temperature of water bath is 30° and the holding time is1 hour; then, mixing the pectinase and laccase into complex enzyme,wherein the weight proportion of pectinase and laccase is 9:1, and theweight proportion of such complex enzyme and the jute fibres is 5:100;next, diluting the complex enzyme with water, in order to producecomplex enzyme water solution which is 40 times in weight as much as thejute fibres; after that, soaking the jute fibres in the diluted complexenzyme water solution; then, adjusting the PH value of the dilutedcomplex enzyme water solution to 4.9 with acetic acid and sodiumbicarbonate; next, heating the complex enzyme water solution to 40° C.and keeping the solution at such temperature for 120 minutes; afterthat, adjusting the PH value of the heated solution to 9.5 with sodiumbicarbonate, heating the solution to 55° C., and keeping the solution atsuch temperature for 40 minutes; then, taking the jute fibres out of thesolution, and accumulation storing the jute fibres for 6 hours; next,conducting enzyme deactivation of the accumulation stored jute fibres bywashing the jute fibres with hot water at 95° C.; finally, the degummedjute fibres are obtained. The removal rate of pectin and lignin fromjute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 3

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through acid bath, wherein the acid used for acid bath isacetic acid with the concentration of above 90%, then, mixing thepectinase and laccase into complex enzyme, wherein the weight proportionof pectinase and laccase is 1:1, and the weight proportion of suchcomplex enzyme and the jute fibres is 1:100; next, diluting the complexenzyme with water, in order to produce complex enzyme water solutionwhich is 15 times in weight as much as the jute fibres; after that,soaking the jute fibres in the diluted complex enzyme water solution;then, adjusting the PH value of the diluted complex enzyme watersolution to 4.6 with acetic acid and sodium bicarbonate; next, heatingthe complex enzyme water solution to 55° C. and keeping the solution atsuch temperature for 40 minutes; after that, adjusting the PH value ofthe heated solution to 8.5 with sodium bicarbonate, heating the solutionto 50° C., and keeping the solution at such temperature for 50 minutes;then, taking the jute fibres out of the solution, and accumulationstoring the jute fibres for 10 hours; next, conducting enzymedeactivation of the accumulation stored jute fibres by washing the jutefibres with hot water at 85° C.; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 4

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through soaking the jute fibres in hydrogen peroxide withthe concentration of 5 g/L, then, mixing the pectinase and laccase intocomplex enzyme, wherein the weight proportion of pectinase and laccaseis 3:1, and the weight proportion of such complex enzyme and the jutefibres is 2:100; next, diluting the complex enzyme with water, in orderto produce complex enzyme water solution which is 30 times in weight asmuch as the jute fibres; after that, soaking the jute fibres in thediluted complex enzyme water solution; then, adjusting the PH value ofthe diluted complex enzyme water solution to 4.8 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to50° C. and keeping the solution at such temperature for 50 minutes;after that, adjusting the PH value of the heated solution to 9.0 withsodium bicarbonate, heating the solution to 60° C., and keeping thesolution at such temperature for 90 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for15 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with hot water at 90° C.;finally, the degummed jute fibres are obtained. The removal rate ofpectin and lignin from jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 5

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through water bath at the temperature of 100 L, and theholding time is half an hours; then, mixing the pectinase and laccaseinto complex enzyme, wherein the weight proportion of pectinase andlaccase is 5:1, and the weight proportion of such complex enzyme and thejute fibres is 3:100; next, diluting the complex enzyme with water, inorder to produce complex enzyme water solution which is 20 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 4.1 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to45° C. and keeping the solution at such temperature for 60 minutes;after that, adjusting the PH value of the heated solution to 8.5 withsodium bicarbonate, heating the solution to 40° C., and keeping thesolution at such temperature for 70 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for20 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with water solution, thePH value of which is 11.0; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 6

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 4:1, and the weight proportion of such complex enzyme andthe jute fibres is 4:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 16 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 4.2 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to50° C. and keeping the solution at such temperature for 70 minutes;after that, adjusting the PH value of the heated solution to 9.0 withsodium bicarbonate, heating the solution to 45° C., and keeping thesolution at such temperature for 80 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for12 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with water solution, thePH value of which is 3.0; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 7

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 2:3, and the weight proportion of such complex enzyme andthe jute fibres is 1:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 13 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 4.0 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to60° C. and keeping the solution at such temperature for 80 minutes;after that, adjusting the PH value of the heated solution to 8.0 withsodium bicarbonate, heating the solution to 65° C., and keeping thesolution at such temperature for 100 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for8 hours; next, conducting enzyme deactivation of the accumulation storedjute fibres by washing the jute fibres with hot water at 85° C.;finally, the degummed jute fibres are obtained. The removal rate ofpectin and lignin from jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 8

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 3:1, and the weight proportion of such complex enzyme andthe jute fibres is 2:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 14 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 4.5 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to65° C. and keeping the solution at such temperature for 90 minutes;after that, adjusting the PH value of the heated solution to 7.8 withsodium bicarbonate, heating the solution to 40° C., and keeping thesolution at such temperature for 110 minutes; then, taking the jutefibres out of the solution; next, conducting enzyme deactivation of thejute fibres by washing the jute fibres with hot water, the PH value ofwhich is 10.0 and the temperature of which is 75° C.; finally, thedegummed jute fibres are obtained. The removal rate of pectin and ligninfrom jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 9

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 2:1, and the weight proportion of such complex enzyme andthe jute fibres is 1:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 16 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 4.3 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to45° C. and keeping the solution at such temperature for 100 minutes;after that, adjusting the PH value of the heated solution to 9.3 withsodium bicarbonate, heating the solution to 55° C., and keeping thesolution at such temperature for 120 minutes; then, taking the jutefibres out of the solution; next, conducting enzyme deactivation of thejute fibres by washing the jute fibres with hot water, the PH value ofwhich is 3.5 and the temperature of which is 80° C.; finally, thedegummed jute fibres are obtained. The removal rate of pectin and ligninfrom jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 10

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through water bath at the temperature of 65°, and theholding time is 2 hours; then, mixing the pectinase and laccase intocomplex enzyme, wherein the weight proportion of pectinase and laccaseis 3:7, and the weight proportion of such complex enzyme and the jutefibres is 0.5:100; next, diluting the complex enzyme with water, inorder to produce complex enzyme water solution which is 12 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.5 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to55° C. and keeping the solution at such temperature for 51 minutes;after that, adjusting the PH value of the heated solution to 7.5 withsodium bicarbonate, heating the solution to 60° C., and keeping thesolution at such temperature for 20 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for24 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with hot water at 80° C.;finally, the degummed jute fibres are obtained. The removal rate ofpectin and lignin from jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 11

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through both acid bath and water bath, wherein the acid usedfor acid bath is concentrated sulphuric acid with the concentration ofabove 90%. The temperature of water bath is 30° and the holding time is1 hour; then, mixing the pectinase and laccase into complex enzyme,wherein the weight proportion of pectinase and laccase is 9:1, and theweight proportion of such complex enzyme and the jute fibres is 5:100;next, diluting the complex enzyme with water, in order to producecomplex enzyme water solution which is 40 times in weight as much as thejute fibres; after that, soaking the jute fibres in the diluted complexenzyme water solution; then, adjusting the PH value of the dilutedcomplex enzyme water solution to 5.0 with acetic acid and sodiumbicarbonate; next, heating the complex enzyme water solution to 60° C.and keeping the solution at such temperature for 120 minutes; afterthat, adjusting the PH value of the heated solution to 7.5 with sodiumbicarbonate, heating the solution to 65° C., and keeping the solution atsuch temperature for 40 minutes; then, taking the jute fibres out of thesolution, and accumulation storing the jute fibres for 6 hours; next,conducting enzyme deactivation of the accumulation stored jute fibres bywashing the jute fibres with hot water at 95° C.; finally, the degummedjute fibres are obtained. The removal rate of pectin and lignin fromjute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 12

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through both acid bath, wherein the acid used for acid bathis acetic acid with the concentration of above 90%, then, mixing thepectinase and laccase into complex enzyme, wherein the weight proportionof pectinase and laccase is 1:1, and the weight proportion of suchcomplex enzyme and the jute fibres is 1:100; next, diluting the complexenzyme with water, in order to produce complex enzyme water solutionwhich is 20 times in weight as much as the jute fibres; after that,soaking the jute fibres in the diluted complex enzyme water solution;then, adjusting the PH value of the diluted complex enzyme watersolution to 5.0 with acetic acid and sodium bicarbonate; next, heatingthe complex enzyme water solution to 55° C. and keeping the solution atsuch temperature for 60 minutes; after that, adjusting the PH value ofthe heated solution to 8.0 with sodium bicarbonate, heating the solutionto 70° C., and keeping the solution at such temperature for 50 minutes;then, taking the jute fibres out of the solution, and accumulationstoring the jute fibres for 10 hours; next, conducting enzymedeactivation of the accumulation stored jute fibres by washing the jutefibres with hot water at 85° C.; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 13

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through soaking the jute fibres in hydrogen peroxide withthe concentration of 5 g/L, then, mixing the pectinase and laccase intocomplex enzyme, wherein the weight proportion of pectinase and laccaseis 2:1, and the weight proportion of such complex enzyme and the jutefibres is 2:100; next, diluting the complex enzyme with water, in orderto produce complex enzyme water solution which is 30 times in weight asmuch as the jute fibres; after that, soaking the jute fibres in thediluted complex enzyme water solution; then, adjusting the PH value ofthe diluted complex enzyme water solution to 5.3 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to58° C. and keeping the solution at such temperature for 90 minutes;after that, adjusting the PH value of the heated solution to 7.8 withsodium bicarbonate, heating the solution to 69° C., and keeping thesolution at such temperature for 90 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for15 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with hot water at 90° C.;finally, the degummed jute fibres are obtained. The removal rate ofpectin and lignin from jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 14

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, pre-processing the bits ofjute fibres through water bath at the temperature of 100°, and theholding time is half an hours; then, mixing the pectinase and laccaseinto complex enzyme, wherein the weight proportion of pectinase andlaccase is 5:1, and the weight proportion of such complex enzyme and thejute fibres is 3:100; next, diluting the complex enzyme with water, inorder to produce complex enzyme water solution which is 12 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.0 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to55° C. and keeping the solution at such temperature for 80 minutes;after that, adjusting the PH value of the heated solution to 7.7 withsodium bicarbonate, heating the solution to 65° C., and keeping thesolution at such temperature for 70 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for20 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with water solution, thePH value of which is 11.0; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 15

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 4:1, and the weight proportion of such complex enzyme andthe jute fibres is 4:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 14 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.0 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to60° C. and keeping the solution at such temperature for 70 minutes;after that, adjusting the PH value of the heated solution to 8.0 withsodium bicarbonate, heating the solution to 65° C., and keeping thesolution at such temperature for 80 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for12 hours; next, conducting enzyme deactivation of the accumulationstored jute fibres by washing the jute fibres with water solution, thePH value of which is 3.0; finally, the degummed jute fibres areobtained. The removal rate of pectin and lignin from jute fibres isindicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 16

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 2:3, and the weight proportion of such complex enzyme andthe jute fibres is 1:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 13 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.2 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to57° C. and keeping the solution at such temperature for 100 minutes;after that, adjusting the PH value of the heated solution to 8.0 withsodium bicarbonate, heating the solution to 60° C., and keeping thesolution at such temperature for 100 minutes; then, taking the jutefibres out of the solution, and accumulation storing the jute fibres for8 hours; next, conducting enzyme deactivation of the accumulation storedjute fibres by washing the jute fibres with hot water at 85° C.;finally, the degummed jute fibres are obtained. The removal rate ofpectin and lignin from jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 17

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 3:1, and the weight proportion of such complex enzyme andthe jute fibres is 2:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 13 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.0 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to58° C. and keeping the solution at such temperature for 110 minutes;after that, adjusting the PH value of the heated solution to 7.8 withsodium bicarbonate, heating the solution to 70° C., and keeping thesolution at such temperature for 110 minutes; then, taking the jutefibres out of the solution; next, conducting enzyme deactivation of thejute fibres by washing the jute fibres with hot water, the PH value ofwhich is 10.0 and the temperature of which is 75° C.; finally, thedegummed jute fibres are obtained. The removal rate of pectin and ligninfrom jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

Example 18

An experiment is conducted through the following steps: firstly,dividing the jute fibres into several bits, wherein each bit of jutefibres weighs about 0.5 kilogram; secondly, mixing the pectinase andlaccase into complex enzyme, wherein the weight proportion of pectinaseand laccase is 2:1, and the weight proportion of such complex enzyme andthe jute fibres is 1:100; next, diluting the complex enzyme with water,in order to produce complex enzyme water solution which is 16 times inweight as much as jute fibres; after that, soaking the jute fibres inthe diluted complex enzyme water solution; then, adjusting the PH valueof the diluted complex enzyme water solution to 5.4 with acetic acid andsodium bicarbonate; next, heating the complex enzyme water solution to56° C. and keeping the solution at such temperature for 100 minutes;after that, adjusting the PH value of the heated solution to 7.6 withsodium bicarbonate, heating the solution to 55° C., and keeping thesolution at such temperature for 120 minutes; then, taking the jutefibres out of the solution; next, conducting enzyme deactivation of thejute fibres by washing the jute fibres with hot water, the PH value ofwhich is 3.5 and the temperature of which is 80° C.; finally, thedegummed jute fibres are obtained. The removal rate of pectin and ligninfrom jute fibres is indicated in the table 1.

Said degummed jute fibres will be highly spinnable, after beingbleached, stamped, washed, dehydrated, and dried via the prior art.

The pectinase (Bioprep) and the laccase (Denilite) mentioned in aboveexamples are produced by the a Danish company called Novozymes. Table 1illustrates the removal rates of pectin and lignin from jute fibres ofthe different examples.

While this invention has been described as having several preferredembodiments, the present invention can be further modified within thespirit and scope of this disclosure. This application is thereforeintended to cover any variations, uses, or adaptations of the inventionusing its general principles. Further, this application is intended tocover such departures from this present disclosure as come within knownor customary practice in the art to which this invention pertains andwhich fall within the limits of the appended claims.

TABLE 1 Examples 1 2 3 4 5 6 7 8 9 Removal rate 86% 96% 90% 92% 95% 96%92% 91% 89% of pectinase Removal rate 79% 79% 80% 80% 84% 86% 82% 81%78% laccase Examples 10 11 12 13 14 15 16 17 18 Removal rate 86% 96% 90%90% 95% 96% 91% 91% 89% of pectinase Removal rate 79% 80% 79% 80% 84%86% 82% 81% 79% laccase

1. A method of degumming jute fibres with complex enzyme, wherein saidcomplex enzyme comprises pectinase and laccase, comprising the steps of:a. soaking the jute fibres in a water solution of said complex enzymemade from pectinase and laccase, wherein the weight proportion of saidcomplex enzyme water solution and jute fibres ranges from 12:1 to 40:1.b. adjusting the pH value of said complex enzyme water solution to above4.0, but below 5.0, and adjusting the temperature of said complex enzymewater solution to 35° C.-65° C., then keeping said complex enzyme watersolution with such temperature value for 20-120 minutes. c. adjustingthe pH value of said complex enzyme water solution to 7.5-9.5, andadjusting the temperature of said complex enzyme water solution to 40°C.-70° C.; then, keeping said complex enzyme water solution at suchtemperature for 20-120 minutes. d. conducting enzyme deactivation of thejute fibres processed with said complex enzyme.
 2. The method of claim1, wherein said jute fibres are accumulation stored before the step d.3. The method of claim 2, wherein duration of accumulation storing saidjute fibres ranges from 6 to 24 hours.
 4. The method of claim 1, whereinthe enzyme deactivation of jute fibres in step d is through washing withhot water or adjusting the pH value of jute fibres, or through thecombination of the two means.
 5. The method of claim 1, wherein theweight percentage of pectinase in said complex enzyme ranges from 30% to90%.
 6. The method of claim 1, wherein the weight proportion of saidcomplex enzyme and jute fibres ranges from 0.5:100 to 5:100.
 7. Themethod of claim 4, wherein the temperature of said hot water is above75°; the pH value of jute fibres is adjusted to above 10.0 or below 4.0.8. The method of claim 1, wherein said jute fibres is pre-processedbefore the step a.
 9. The method of claim 8, wherein the pre-processingof said jute fibres is either through one of the means of water bath,acid bath, or soaking with hydrogen peroxide, or through the combinationof at least two of the three means.
 10. The method of claim 9, whereinthe temperature of the water bath ranges from 30° C. to 100° C.; saidacid is sulphuric acid or acetic acid.
 11. A method of degumming jutefibres with complex enzyme, wherein said complex enzyme comprisespectinase and laccase, comprising the steps of: a. soaking the jutefibres in a water solution of said complex enzyme made from pectinaseand laccase, wherein the weight proportion of said complex enzyme watersolution and jute fibres ranges from 12:1 to 40:1. b. adjusting the pHvalue of said complex enzyme water solution to 5.0-5.5, and adjustingthe temperature of said complex enzyme water solution to 55° C.-60° C.,then keeping said complex enzyme water solution with such temperaturevalue for 51-120 minutes. c. adjusting the pH value of said complexenzyme water solution to 7.5-8.0, and adjusting the temperature of saidcomplex enzyme water solution to 60° C.-70° C.; then, keeping saidcomplex enzyme water solution with such temperature value for 20-120minutes. d. conducting enzyme deactivation of the jute fibres processedwith said complex enzyme.
 12. The method of claim 11, wherein said jutefibres are accumulation stored before the step d.
 13. The method ofclaim 12, wherein the duration for accumulation storing said jute fibresranges from 6 to 24 hours.
 14. The method of claim 11, wherein theenzyme deactivation of jute fibres in step d is through washing with hotwater or adjusting pH value of jute fibres, or through the both of thetwo means.
 15. The method of claim 11, wherein the weight percentage ofpectinase in said complex enzyme ranges from 30% to 90%.
 16. The methodof claim 11, wherein the weight proportion of said complex enzyme andjute fibres ranges from 0.5:100 to 5:100.
 17. The method of claim 14,wherein the temperature of said hot water is above 75° C.; the pH valueof jute fibres is adjusted to above 10.0 or below 4.0.
 18. The method ofclaim 11, wherein said jute fibres are pre-processed before step a. 19.The method of claim 18, wherein pre-processing of said jute fibres iseither through one of the means of water bath, acid bath, or soakingwith hydrogen peroxide, or through at least two of the three means. 20.The method of claim 19, wherein the temperature of water bath rangesfrom 30° C. to 100° C.; said acid is sulphuric acid or acetic acid.